ContentsPreparation
Choose Input FilesUse the menu function The File Browser on the left shows a tree with all sub-directories of a root directory that is shown in the box above the tree.
The DNA Sequence files in SCF, ABI or FASTA file format are accepted.
By default only chromatogram files are shown in the browser. Use the Assembling PreviewThe Assembling Preview on the right side is used to arrange reads into different Samples, Task Entries and targets before performing the assembling. The Use the file browser to navigate to your sequence files or to an upper directory, and select the files or the directory to which they belong to. Sort Reads into TargetsPress the button A new dialog window will appear. Select the Project to which the sequences should be added. The task selection can normally be left to automatically. According to the file naming conventions, the software now tries to sort the read files into Samples, Task Entries and targets. The result is shown in the preview table: each row stands for one read file. By default the file naming conventions are taken from the selected Task Template. To use other file naming conventions, select Match by ad hock File Naming and press the Create File Naming button to define a new file naming on the fly. If the sorting was successful, click the OK-button to create the previewed Samples. Control the ResultThe assembling preview panel shows a preview of the Samples to which the sorted reads will be added. If the sample name that was extracted from the file name of a read file matches an existing Sample in the selected Project, then the read is added to this Sample. Read files can thus be added to existing Samples by using corresponding file names. The result list on the right shows the newly added reads highlighted yellow, and the already existing reads highlighted blue. Clicking the OK-button starts the assembling process and opens the Samples in the main window. |