Ports and Services

The communication between SeqSphere+ Client and SeqSphere+ Server runs on port 8064 (port can be changed). The server and client are communicating via https using a self-signed certificate that is created when the server is started for the first time (SHA-256 with RSA encrypting, 2048 bit).

For some (optional) functions the SeqSphere+ Client needs an Internet connection. The following hosts are connected:

Upload

Function	Host	Protocol, Port
Upload/download for SeqSphere+ license activation, alternatively activation by email possible	act.ridom.de	https, 443
Upload of alleles and optional metadata for cgMLST.org submissions and download of task templates	nomenclature.seqsphere.de	https, 443
Upload of spa-types and optional metadata for S. aureus spa-typing and download of spa-types	spa.ridom.de	https, 443
Upload of contigs for SARS-CoV-2 PANGO lineage typing The server does not store any result or sequence data	tools.ridom.de	https, 443
Upload of meta data for EBI ENA submission	www.ebi.ac.uk	https, 443
Upload of read data for EBI ENA submission (via FTP or Aspera)	webin.ebi.ac.uk	ftp, 21 or TCP/UDP, 33001

Download

Function	Host(s)	Protocol, Port
Download of SeqSphere+ software updates, index files for NCBI Genome Browser, and PubMLST.org MLST schemes	www.ridom.de	https, 443
Download of genomes from NCBI Genome	www.ncbi.nlm.nih.gov	https, 443
Download of read data from NCBI SRA	trace.ncbi.nlm.nih.gov, sra-pub-run-odp.s3.amazonaws.com	https, 443
Download of MLST schemes from PubMLST.org	pubmlst.org, rest.pubmlst.org	https, 443
Geocoding with GeoNames	www.geonames.org	http, 80

Additional Download required only for Linux

Function	Host(s)	Protocol, Port
Download of MOB-Suite database on Linux	zenodo.org	https, 443

The SeqSphere+ Server does not require any Internet connection.

Submission to cgMLST.org

The cgMLST.org Nomenclature Server (www.cgMLST.org) provides a global nomenclature for stable public cgMLST schemes, i.e. for Task Templates that were downloaded from the Task Template Sphere. If Samples are using those downloaded Task Templates they can be submitted to cgMLST.org.

If no submission of any data is wanted or allowed, then only local Task Templates must be used. Samples that are using only local Task Templates will not and cannot be submitted to cgMLST.org. Local Task Templates can be defined using the cgMLST Target Definer, or by converting public Task Templates into local ones.

Remote Access

By installing SeqSphere+, Ridom has by no means remote access to the user's local computer or SeqSphere+ database. Remote access for training and support is usually done with Microsoft Teams.