High interlaboratory reproducibility and accuracy of NGS based bacterial genotyping in a ring trial
SeqSphere+ was used in an international bacterial genomic typing ring trial with high reproducibility and accuracy (JCM 55: 908, 2017).
Real time genome sequencing with SeqSphere+ of resistant bacteria provides precision infection control in an institutional setting
The increasing prevalence of multidrug-resistant (MDR) bacteria is a serious global challenge. In a just published study (J. Clin. Microbiol. 54: 2874, 2016) it was prospectively analyzed whether bacterial whole-genome sequencing (WGS) for real-time MDR surveillance is technical feasible, returns actionable results, and is cost-beneficial. WGS was applied to all MDR isolates of four species (methicillin-resistant Staphylococcus aureus [MRSA], vancomycin-resistant Enterococcus faecium, MDR Escherichia coli, and MDR Pseudomonas aeruginosa) at the University Hospital Muenster, Muenster, Germany, a tertiary care hospital with 1,450 beds, during two 6-month intervals. More than 1,200 isolate WGS data were analyzed automatically with the Ridom SeqSphere+ tool. Turnaround times (TAT) were measured, and total costs for sequencing per isolate were calculated. After cancelling prior policies of preemptive isolation of patients harboring certain Gram-negative MDR bacteria in risk areas, the second interval was conducted. During interval I, 645 bacterial isolates were sequenced. From culture, TATs ranged from 4.4 to 5.3 days, and costs were €202.49 per isolate. During interval II, 550 bacterial isolates were sequenced. Hospital-wide transmission rates of the two most common species (MRSA and MDR E. coli) were low during interval I (5.8% and 2.3%, respectively) and interval II (4.3% and 5.0%, respectively). Cancellation of isolation of patients infected with non-pan-resistant MDR E. coli in risk wards did not increase transmission. Comparing sequencing costs with avoided costs mostly due to fewer blocked beds during interval II, €200,000 in excess was saved. Real-time microbial WGS in our institution was feasible, produced precise actionable results, helped to monitor transmission rates that remained low following a modification in isolation procedures, and ultimately saved costs.
Meningococcal disease: identification of a sexually transmitted variant
A study elucidates the mechanisms of the emergence of an outbreak of meningococcal disease in men who have sex with men (MSM).
SeqSphere+ Version 3.1 released that is freely available to customers
This is a maintenance release with a number of smaller improvements and bug fixes (e.g., EBI ENA submission works now again after EBI has changed some details of the API; proxy support for SRA download). Most important the cgMLST Target Definer and our NCBI Genome Browser now support bacteria with multiple chromosomes. In addition draft genomes can be now chosen if really needed in the Target Definer as reference genomes. All NCBI codon translation tables are now properly supported on various places in the SeqSphere+ software. Furthermore, export of core and/or accessory genome gene concatenated SNPs in FASTA file format is now supported. Finally, also export of trees in publication ready EMF format that immediately can be read by e.g. MS PowerPoint is now implemented. For a full list of version changes invoke the SeqSphere+ menu command Help | About.
SeqSphere+ version 3.0 released that is freely available to customers with GIS & epi-curve functionality and SRA interface
Most important we have now implemented geographical information system (GIS) and epi-curve functionality for visualization of the four dimensions place, time, ‘person’, and type. Furthermore, a convenient download of NCBI complete and draft genomes and raw reads from the Sequence Read Archive (SRA) has been realized. Finally, a first version of sample reports has been incorporated as major new feature. In addition, bug-fixes and many smaller usability enhancements are part of this release.
Novel core-genome MLST typing scheme for Listeria monocytogenes presents prospects for a standardized,
portable and expandable typing method based on whole genome sequencing.
SeqSphere+ was used to establish and evaluate a cgMLST scheme for L. monocytogenes.(JCM 53: 2015)
Novel core-genome MLST typing scheme for Legionella pneumophilia presents prospects for a standardized,
portable and expandable typing method based on whole genome sequencing.
SeqSphere+ was used to establish and evaluate a cgMLST scheme for L. pneumophilia. (Eurosurveillance 20: 2015)
SeqSphere+ Version 2.3 with Raw Read Data Submission to EBI ENA Released
As more and more of our customers start using the software on a daily basis for routine surveillance tasks we laid great emphasis in implementing productivity enhancement features.
In more than 99% of all S. aureus samples the correct Spa type could be extracted by Ridom SeqSphere+ from WGS data
From 423 MRSA strains encoding 75 different spa types (with 2-19 repeats) the correct Spa type could be extracted in 99.1% from WGS data (250bp Nextera XT read pairs; SeqSphere+ analysis with incorporated Velvet assembly) (CMI 20: 2014).
Article in Press...
Review: WGS Analysis and Interpretation in Clinical and Public Health Microbiology Laboratories
Review 'WGS Analysis and Interpretation in Clinical and Public Health Microbiology Laboratories: What Are the Requirements and How Do Existing Tools Compare?' just published that includes Ridom SeqSphere+ among other tools. (Pathogens 3: 2014).
Whole genome based Mycobacterium tuberculosis surveillance: A standardized, portable, and expandable approach
A new study published in Journal Clinical Microbiology demonstrated that standardization of whole genome sequencing based (WGS) genotyping of the bacteria causing tuberculosis (TB) is possible, and can be used for improved tracing of pathogen transmission (JCM 52: 2014).
MRSA whole genome typing with Ridom SeqSphere+ and MLST+
Bacterial whole genome sequencing revisited: portable, scalable and standardized analysis for typing and detection of virulence and antibiotic resistance genes (JCM 52: 2014).
Listeria whole genome typing with Ridom SeqSphere+ and MLST+
Whole genome sequencing as a tool to investigate a cluster of seven cases of listeriosis in Austria and Germany, 2011-2013. (CMI 20: 2014).
Life Technologies released App-note describing Ridom SeqSphere+
Life Technologies Corporation (NASDAQ: LIFE) just has released an App-note describing bacterial typing using the Ion PGM™ system and Ridom SeqSphere+ software.
Dag Harmsen discusses the advantage of benchtop sequencing for smaller laboratories
He believes that the afforability of benchtop sequencers, such as, the Ion PGM™ Sequencer is democratizing next generation sequencing, allowing smaller laboratories to compete with larger genome sequencing centers.
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Life Technologies Partners with Ridom and Releases SeqSphere+ Software to Speed and Simplify Bacterial Typing
Life Technologies Corporation announced that it is partnering with Ridom GmbH (Münster, Germany) to make SeqSphere+ whole genome bacterial typing software available for Ion Torrent users.
Update on next generation benchtop sequencing - Ridom SeqSphere+ software used for analysis
New study "Updating benchtop sequencing performance comparison" published in the Nature Biotechnology journal / MiSeq and PGM mature and affordable enough for soon application in routine epidemiologic surveillance for clinical and public health microbiology.
Rapid NGS for public health microbiology workshop
The in cooperation with PathoNGenTrace organized Workshop & Conference Rapid NGS for Public Health Microbiology was held successfully in Münster, Germany from March 4th to 8th 2013. About 80 participants from 20 countries attended the one day conference. 26 participants stayed for the whole week for a wet laboratory - Ion Torrent PGM and Illumina MiSeq bacterial whole genome sequencing - and computer class part (CLC Genomics Workbench, BIGSdb, Outbreaker, Ridom SeqSphere+ and CloVR).
Ridom celebrates 10-year anniversary
Founded January 2003 Ridom GmbH (Münster, Germany) celebrates its ten year anniversary. We are proud due to the loyal and continuous support of our customers to serve the clinical and public health microbiology community for such a long time. Stay another ten years with us and watch out for 'cool' new software products to be released soon.
Patho-NGen-Trace project With Ridom as consortium member started
The Patho-NGen-Trace project, proposed to the FP7 (Seventh Framework Programme for Research and Technological Development), has been started. The overall objective of Patho-NGen-Trace is to foster the development of new and widespread applications of Next Generation Sequencing (NGS) in clinical microbiology and disease surveillance, ranging from basic research to medical research, diagnostics, and pathogen genotyping. Major aim of Patho-NGen-Trace is to bring NGS from a basic research tool to a highly efficient technology for pathogen typing and diagnostics on the EU level.